Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The EC50 this effect is less than 1μg/ml in the presence of 1.5 μg/ml recombinant human Klotho β.
FGF-21 Protein, Human
FGF-21 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | FGF-21;Fibroblast Growth Factor 21, FGF-21, FGF21 |
| Accession | Q9NSA1-1 |
| Amino Acid Sequence | His29-Ser209, with N-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 25-28 KDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.2, 5% trehalose |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. According to its structure, it is further classified as a member of the FGF19 subfamily. The subfamily includes FGF-19,-21 and-23. FGF-21 is an effective adipocyte glucose uptake activator that protects animals from diet-induced obesity when overexpressed in transgenic mice and reduces blood glucose and triglyceride levels during treatment in diabetic rodents. Some studies have shown that FGF-21 is a biomarker for the diagnosis of mitochondrial diseases. FGF-21 can cross the blood-brain barrier and regulate the phosphorylation cascade and gene expression in the whole hypothalamus. Intracerebroventricular injection of FGF21 can increase metabolic rate and insulin sensitivity in rats, but the site of action is not clear.
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Bioactivity
SDS-PAGE
FGF-21 His tag, Human，2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.
RP-HPLC
