Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells.The EC50 for this effect is less than 1.5ng/ml.
EGF Protein, Bovine (Animal Free)
EGF Protein, Bovine (Animal Free)
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Product Details
Product Details
Product Specification
| Species | Bovine |
| Synonyms | Pro-epidermal growth factor; EGF; |
| Accession | XP_059743690 |
| Amino Acid Sequence | Lys985-His1037 |
| Expression System | E.coli |
| Molecular Weight | 6 kDa (Reducing) |
| Purity | >95% by SDS-PAG. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 300mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Science. 1991 Feb 22;251(4996):936-9. |
Background
Epidermal Growth Factor (EGF) is a protein that acts as a potent mitogen and stimulator of cell proliferation. EGF is a growth factor that plays a crucial role in various biological processes, including cell growth, differentiation, and tissue repair. EGF binds to the EGF receptor (EGFR) on the cell surface, leading to the activation of downstream signaling pathways such as the MAPK/ERK pathway and the PI3K/Akt pathway. In bovine species, EGF has been studied for its effects on cell growth and development, particularly in the context of wound healing and tissue regeneration. Bovine EGF is commonly used in research and biotechnology applications to study cell signaling, proliferation, and tissue repair mechanisms.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
