Des1-3 IGF1 Protein, Human
Des1-3 IGF1 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF,Des1-3 Insulin-like Growth factor-1 |
| Accession | P05019 |
| Amino Acid Sequence | Thr49-Ala118 |
| Expression System | E.coli |
| Molecular Weight | Approximately 7.4 kDa, a non-glycosylated polypeptide chain containing 67 amino acids. |
| Purity | ＞98% by SDS-PAGE or HPLC. |
| Endotoxin | <0.01EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20 mM PBS, pH 7.0. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity. Des1-3IGF-1, is a truncated variant of human IGF-1 with the tripeptide Gly-Pro-Glu absent from the N-terminus. The des1-3IGF-1 probably results from post-translational cleavage of IGF-1. It has about 10-fold more potent than IGF-1 at stimulating hypertrophy and proliferation of cultured cells, a consequence of much reduced binding to IGF-binding proteins, in turn caused by the absence of the glutamate at position 3. Recombinant human des1-3IGF-1 is a non-glycosylated polypeptide chain containing 67 amino acids..
