WB result of ZP3 Recombinant Rabbit mAb
Primary antibody: ZP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Mouse ovary lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/1ZP0000 dilution
Predicted MW: 47 kDa
Observed MW: 80 kDa
ZP3 Recombinant Rabbit mAb (SDT-2201-35)
ZP3 Recombinant Rabbit mAb (SDT-2201-35)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ZP3 |
| Synonyms | Zona pellucida sperm-binding protein 3; Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3 (Zp-3); Zona pellucida protein C; ZP3A; ZP3B; ZPC |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P21754 |
| Clone Number | SDT-2201-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF, WB |
| Reactivity | Ms, Rt |
| Positive Sample | Mouse ovary, rat ovary |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:1000 | Ms, Rt |
Background
ZP3, also known as zona pellucida glycoprotein 3 or sperm receptor, is a glycoprotein encoded by the ZP3 gene and is a major structural component of the zona pellucida, an extracellular matrix that surrounds the oocyte and early embryo. It is crucial for sperm binding and induction of the acrosome reaction, which is essential for fertilization. In mice, ZP3 is the single zona pellucida protein sufficient and necessary for sperm binding in vitro, though it is insufficient for fertilization in vivo. In humans, ZP1, ZP3, and ZP4 all appear partially responsible for starting the acrosome reaction. The nascent ZP3 protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. Furin cleavage is hypothesized to release the mature protein from the plasma membrane for incorporation into the zona pellucida matrix.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse ovary. Anti-ZP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat ovary. Anti-ZP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse ovary. Anti-ZP3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat ovary. Anti-ZP3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
