TMEM16A Recombinant Rabbit mAb (SDT-2179-12)
TMEM16A Recombinant Rabbit mAb (SDT-2179-12)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TMEM16A |
| Synonyms | Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2; DOG1; ORAOV2; TAOS2; ANO1 |
| Immunogen | Recombinant Protein |
| Location | Apical cell membrane |
| Accession | Q5XXA6 |
| Clone Number | SDT-2179-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
TMEM16A, also known as anoctamin 1, is a multifunctional protein that plays a crucial role in cellular physiology. It functions as a calcium-activated chloride channel, facilitating the transport of chloride ions across cell membranes in response to increases in intracellular calcium levels. This ion channel activity is essential for various physiological processes, including the regulation of membrane potential, cell volume, and the secretion of fluids in epithelial tissues. TMEM16A is widely expressed in different tissues such as the respiratory tract, gastrointestinal tract, and cardiovascular system. Additionally, it has been implicated in the pathogenesis of certain diseases like cystic fibrosis and cancer, making it a potential therapeutic target for the development of new treatments.
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Western Blot
WB result of TMEM16A Recombinant Rabbit mAb
Primary antibody: TMEM16A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Lane 5: Capan-1 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 114 kDa
Observed MW: 120~160kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human gastrointestinal stromal tumor (case 1). Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastrointestinal stromal tumor (case 2). Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human synovial sarcoma. Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human leiomyosarcoma. Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liposarcoma. Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-TMEM16A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
