ICC shows positive staining in HeLa cells. Anti- Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (meganta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Stat6 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-337-32)
Stat6 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-337-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Stat6 |
| Synonyms | Signal transducer and activator of transcription 6; IL-4 Stat; STAT6 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42226 |
| Clone Number | SDT-337-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF, ICFCM |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | Hu |
| IF | 1:200 | Hu |
| ICFCM | 1:500 |
Background
Stat6 protein, which stands for Signal Transducer and Activator of Transcription 6, is a crucial component in the immune system. It is a member of the Stat family of transcription factors that play key roles in transmitting signals from cytokine receptors to the nucleus. When activated by cytokines like IL-4 and IL-13, Stat6 translocates to the nucleus and regulates the expression of numerous genes involved in immune responses, such as those related to the differentiation of T helper 2 cells and the production of immunoglobulin E. This protein is essential for mounting effective immune responses against certain pathogens and also has implications in various immune-related diseases, including asthma and allergic reactions.
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Immunocytochemistry
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human kidney. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
