WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDa
PRDM1/Blimp1 Recombinant Rabbit mAb (S-1972-3)
PRDM1/Blimp1 Recombinant Rabbit mAb (S-1972-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PRDM1/Blimp1 |
| Synonyms | PR domain zinc finger protein 1; BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1 (PRDI-BF1; PRDI-binding factor 1) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O75626 |
| Clone Number | S-1972-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | THP-1, HeLa, A431, RAW264.7, rat colon |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
PRDM1, also known as Blimp1, is a crucial transcriptional regulator that plays significant roles in various biological processes. It is a zinc finger protein with a complex structure containing multiple functional domains. In the immune system, PRDM1/Blimp1 is essential for the terminal differentiation of B cells into plasma cells, helping to suppress the expression of genes related to B cell proliferation and maintenance of the B cell phenotype, thereby facilitating the transition to plasma cells which are specialized for antibody production. Additionally, it also has important functions in other cell types and developmental contexts, such as influencing the differentiation of certain T cell subsets and participating in the regulation of some developmental processes in tissues like the skin. Its dysregulation has been implicated in various diseases, including certain types of cancers and autoimmune disorders, making it an interesting target for potential therapeutic interventions.
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Western Blot
WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDa
WB result of PRDM1/Blimp1 Recombinant Rabbit mAb
Primary antibody: PRDM1/Blimp1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-PRDM1/Blimp1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti-PRDM1/Blimp1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Raw264.7 cells. Anti-PRDM1/Blimp1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
