Flow cytometric analysis of Human CD123 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 488 Mouse Anti-Human HLA/DR antibody and either PE-Cy7 Mouse IgG2a, κ Isotype Control (Left panel) or SDT PE-Cy7 Mouse Anti-Human CD123 Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Mouse Anti-Human CD123 Antibody (S-R442)
PE-Cy7 Mouse Anti-Human CD123 Antibody (S-R442)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD123 |
| Synonyms | Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3RA; IL3R |
| Location | Cell membrane |
| Accession | P26951 |
| Clone Number | S-R442 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD123, also known as the alpha chain of the interleukin-3 receptor (IL-3Rα), is a membrane-bound protein that plays a crucial role in immune regulation and hematopoiesis. It is overexpressed in various hematologic malignancies, including acute myeloid leukemia (AML), blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute lymphoblastic leukemia (ALL), hairy cell leukemia, and systemic mastocytosis. The CD123 protein consists of three extracellular domains, a single-pass transmembrane domain, and a short intracellular region. It is involved in the growth, proliferation, survival, and differentiation of hematopoietic cells. CD123 is particularly significant as a biomarker for targeted therapy in these diseases, with various pharmacologic strategies and agents under investigation or already approved for clinical use. For instance, Tagraxofusp, an anti-CD123 antibody conjugated to diphtheria toxin, has been approved for use in patients with BPDCN.
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