WB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: LnCap whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
HSP90 Recombinant Mouse mAb (S-5484)
HSP90 Recombinant Mouse mAb (S-5484)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | HSP90 |
| Synonyms | Heat shock protein HSP 90-alpha; Heat shock 86 kDa (HSP 86; HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2; LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; HSP90A; HSPC1; HSPCA; HSP90AA1 |
| Location | Cytoplasm, Nucleus |
| Accession | P07900 |
| Clone Number | S-5484 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, MCF7, Jurkat, LnCap, HepG2, mouse spleen, mouse brain, rat spleen, rat brain, rat testis, rat colon |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
HSP90 (Heat Shock Protein 90) is a highly conserved and extremely abundant molecular chaperone protein within cells. Existing as a homodimer, it assists numerous "client proteins" in proper folding, maturation, and activity maintenance through ATP-dependent conformational changes. These client proteins are predominantly kinases, hormone receptors, and transcription factors involved in cell proliferation, survival, and signal transduction. Its expression is upregulated when cells respond to external stresses such as heat shock and oxidative stress, where it exerts important cytoprotective functions by stabilizing denatured proteins and preventing their aggregation. Additionally, HSP90 participates in immune regulation—for example, by promoting antigen presentation and TLR signaling pathway activation to enhance innate immune responses, as well as supporting T cell proliferation and differentiation. Since its client protein network includes a large number of oncoproteins (such as HER2, AKT, and BRAF), HSP90 inhibition has become an important strategy in cancer therapy. Furthermore, its dysfunction is closely associated with the accumulation of protein aggregates in neurodegenerative diseases (such as Alzheimer's disease) as well as with fungal infection drug resistance.
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Western Blot
WB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of HSP90 Recombinant Mouse mAb
Primary antibody: HSP90 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat colon lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HSP90 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
