Flow cytometric analysis of Human CD11b expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 510™ Mouse Anti-Human CD14 antibody and either APC Rat IgG2b, κ Isotype Control (Left panel) or SDT APC Rat Anti- Human CD11b antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC Rat Anti-Human CD11b Antibody (S-R574)
APC Rat Anti-Human CD11b Antibody (S-R574)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD11b |
| Synonyms | Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Itgam |
| Location | Cell membrane |
| Accession | P05555 |
| Clone Number | S-R574 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD11b, also known as integrin alpha M (ITGAM), is a cell surface receptor belonging to the integrin family and primarily expressed on immune cells such as macrophages, monocytes, neutrophils, and NK cells. It forms a heterodimer with CD18 to create the β2 integrin complex, which is crucial for mediating cell adhesion, migration, and immune responses. CD11b plays a significant role in leukocyte recruitment and activation during inflammation and immune responses by interacting with ligands like ICAM-1 and iC3b. Additionally, it is involved in phagocytosis and immune regulation in various diseases, including cancer and autoimmune conditions.
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