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Actin (plant specific) Rabbit Polyclonal Antibody

Actin (plant specific) Rabbit Polyclonal Antibody

Catalog Number: S0B6046 Application: WB Brand: Starter
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Regular price $100.00 USD
Regular price Sale price $100.00 USD
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Product Details

Product Specification


Host Rabbit
Antigen Actin (plant specific)
Synonyms Actin-2; ACT2
Immunogen Synthetic Peptide
Location Cytoplasm, Cytoskeleton
Accession Q96292
Antibody Type Polyclonal antibody
Isotype IgG
Application WB
Reactivity Rice, Soybean, Arabidopsis
Positive Sample Arabidopsis, rice, soybean
Purification Immunogen Affinity
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Arabidopsis, Rice, Soybean

Background

Actin in plants is a highly conserved cytoskeletal protein that plays essential roles in maintaining cell shape, cytoplasmic streaming, cell division, cell expansion, and intracellular transport. Similar to animal cells, plant actin exists in two forms: monomeric G-actin (globular) and polymeric F-actin (filamentous), which dynamically assemble into microfilament networks to regulate cellular processes. Plants possess multiple actin isoforms (e.g., ACT2, ACT7), each exhibiting tissue-specific and developmental stage-dependent expression patterns—for instance, ACT7 is highly expressed in rapidly growing tissues. Actin filaments interact with various binding proteins, including myosin, formin, and profilin, to drive organelle movement (e.g., chloroplast positioning), cytoplasmic streaming, and critical developmental processes such as cell plate formation, pollen tube tip growth, and stomatal movement. Additionally, plant actin undergoes rapid reorganization in response to environmental stimuli (e.g., gravity, mechanical stress, and pathogen attack), enabling adaptation to external challenges. While the actin gene family is evolutionarily conserved, plants have undergone unique gene duplication and functional diversification to support their distinct growth and developmental strategies.

Picture

Western Blot

WB result of Actin (plant specific) Rabbit pAb
Primary antibody: Actin (plant specific) Rabbit pAb at 1/1000 dilution
Lane 1: arabidopsis whole plant lysate 20 µg
Lane 2: rice whole plant lysate 20 µg
Lane 3: soybean whole plant lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa

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