WB result of Mouse mAb IgG2b, κ Isotype Control
Primary antibody: Mouse mAb IgG2b, κ Isotype Control at 1/100 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Mouse mAb IgG2b, κ Isotype Control (S-844-22)
Mouse mAb IgG2b, κ Isotype Control (S-844-22)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-22 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | WB, IHC-P, FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of Hut 78 (Human Sezary syndrome cutaneous T lymphocyte) labeling Mouse mAb IgG2b, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with CD6 antibody at 1/200 (1 μg) dilution (S0B0379) (Right) / (Red), Mouse monoclonal IgG isotype control (Left)/(Black) compared with Mouse mAb IgG2b, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling Mouse mAb IgG2b, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG isotype control (Left) / (Black) compared with Mouse mAb IgG2b, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Mouse mAb IgG2b, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG isotype control (Left) / (Black) compared with Mouse mAb IgG2b, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows negative staining in paraffin-embedded human tonsil. Mouse mAb IgG2b, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded rat spleen. Mouse mAb IgG2b, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
