Immobilized Mouse IL-12/IL-12A&IL-12B Protein, His tag&No tag at 4 μg/mL (50 μL/well) can bind IL-12 R beta 1 Fc Chimera Protein, Human (Cat. No. UA010956) with EC50 of 9.68-11.89 ng/ml.
Mouse IL-12/IL-12A&IL-12B Protein, His tag&No tag
Mouse IL-12/IL-12A&IL-12B Protein, His tag&No tag
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$200.00 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-12 subunit alpha, Cytotoxic lymphocyte maturation factor 35 kDa subunit (CLMF p35), IL-12 subunit p35, Il12a, Interleukin-12 subunit beta, Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), IL-12 subunit p40, Il12b |
| Accession | P43431、P43432 |
| Amino Acid Sequence | Protein sequence1 (P43431, Arg23-Ala215, with C-His tag)
RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA; |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 23.4 kDa(IL-12A)&35.8 kDa(IL-12B) ; |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | with C-10*His (IL-12A)&No tag (IL-12B) |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, helper T cells and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-β1 and IL-12R-β2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-γ production and killing of target cells. IL-12 also has anti-angiogenic activity.
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Bioactivity
SDS-PAGE
2 μg（R: reducing conditions; NR: non-reducing conditions）
