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Mouse IFN-γ OneStep ELISA Kit

Mouse IFN-γ OneStep ELISA Kit

Catalog Number: S0C3035 Application: ELISA Reactivity: Mouse Conjugation: Brand: Starter
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Regular price $500.00 USD
Regular price Sale price $500.00 USD
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Product Details

Product Specification


Antigen IFN-γ
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Ms
Purification Protein A
Stability & Storage

2 to 8 °C as supplied.

Kit


Precision Intra-assay: 3.6%;
Inter-assay: 6.0%
Sample type Cell culture supernatant, Serum
Assay type Sandwich (quantitative)
Sensitivity 7.398pg/ml
Range 31.25 pg/mL – 2000 pg/mL
Recovery Cell culture supernatant: 83%
Serum: 107%
Assay time 60 minutes
Species reactivity Mouse

Background

Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.

Picture

ELISA

Standard curve
Example of Mouse IFN-γ standard curve in Assay Diluent #9.

Linearity
The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is  undiluted samples are as follows:  CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.

Linearity
The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is  undiluted samples are as follows:  CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.

Protocol Diagram

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