Standard curve
Example of Mouse IFN-γ standard curve in Assay Diluent #9.
Product Details
Product Details
Product Specification
Antigen | IFN-γ |
Immunogen | Recombinant Protein |
Antibody Type | Recombinant mAb |
Reactivity | Ms |
Purification | Protein A |
Stability & Storage | 2 to 8 °C as supplied. |
Kit
Precision | Intra-assay: 3.6%; Inter-assay: 6.0% |
Sample type | Cell culture supernatant, Serum |
Assay type | Sandwich (quantitative) |
Sensitivity | 7.398pg/ml |
Range | 31.25 pg/mL – 2000 pg/mL |
Recovery | Cell culture supernatant: 83% Serum: 107% |
Assay time | 60 minutes |
Species reactivity | Mouse |
Background
Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.
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Picture
ELISA
Linearity
The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.
Linearity
The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.
Protocol Diagram
