Flow cytometric analysis of human peripheral blood labelling Human CD99 antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Mouse IgG2a, κ Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Product Details
Product Details
Product Specification
Host | Mouse |
Antigen | CD99 |
Synonyms | CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; MIC2; MIC2X; MIC2Y; |
Location | Membrane |
Accession | P14209 |
Clone Number | S-R646 |
Antibody Type | Mouse mAb |
Isotype | IgG2a,k |
Application | FCM |
Reactivity | Hu |
Positive Sample | human peripheral blood |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS pH7.4 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
FCM | 1:2000 | Hu |
Background
CD99, also known as MIC2, is a 32 kDa transmembrane glycoprotein encoded by the CD99 gene, located in the pseudoautosomal region of human chromosomes X and Y. It plays crucial roles in various biological processes, including cell adhesion, migration, apoptosis, and immune responses. CD99 is highly expressed in hematopoietic cells, thymus, endothelial cells, and certain cancers. In normal tissues, it is expressed at low levels but shows differential expression in bone marrow and lymphoid cells.
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