Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Human CD40 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ separately. Events were gated on viable lymphocytes.
Mouse Anti-Human CD40 Antibody (S-R530)
Mouse Anti-Human CD40 Antibody (S-R530)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD40 |
| Synonyms | B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40 |
| Location | Secreted, Cell membrane |
| Accession | P25942 |
| Clone Number | S-R530 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Hu |
Background
The CD40 is a type I transgender protein that is transmitted in the immune system's B cells and special antigen to the surface of the cells and many non -hematopoietic cell types and cancer cells. It belongs to the TNF receptor super family.CD40 is expressed in B cells, foam dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. CD40 interacts with CD40 Ligand (CD40L/TNFSF5).CD40/CD40L is essential for the beginning and progress of cells and body fluid adaptive immunity, and it is an important area of concern in the study of tumor immunology, neurodegeneration, vascular disease and inflammatory diseases.
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