Flow cytometric analysis of MOLT-4 (Human embryonic kidney epithelial cell Human lymphoblastic leukemia T lymphoblast, left) / K562 (Human chronic myelogenous leukemia lymphoblast, right) labelling CD235a/b antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: MOLT-4
Mouse Anti-Human CD235a/b Antibody (S-R403)
Mouse Anti-Human CD235a/b Antibody (S-R403)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a/b |
| Synonyms | Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB |
| Location | Cell membrane |
| Accession | P02724、 P06028 |
| Clone Number | S-R403 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | null |
Background
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
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