Flow cytometric analysis of human whole blood cells labelling CD235a antibody at 1/2000 dilution (0.1 μg)/ (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Product Details
Product Details
Product Specification
Host | Mouse |
Antigen | CD235a |
Synonyms | Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GPA, GYPA |
Location | Cell membrane |
Accession | P02724 |
Clone Number | S-R473 |
Antibody Type | Mouse mAb |
Isotype | IgG2a,k |
Application | FCM |
Reactivity | Hu |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
FCM | 1:2000 |
Background
CD235a, also known as Glycophorin A, is the most abundant sialoglycoprotein on the surface of human erythrocytes. It plays a crucial role in reducing red blood cell aggregation and maintains blood fluidity. CD235a serves as a receptor for various pathogens, including the influenza virus, Plasmodium falciparum (the parasite causing malaria), and hepatitis A virus, making it significant in the context of infectious diseases. Its expression and function are essential for the proper physiological function of red blood cells.
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