Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Human CD133 antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45 - PE/Cy7 separately. Gated on total viable cells.
Mouse Anti-Human CD133 Antibody (S-R586)
Mouse Anti-Human CD133 Antibody (S-R586)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD133 |
| Synonyms | Prominin-1; Antigen AC133; Prominin-like protein 1; PROM1; PROML1 |
| Location | Apical cell membrane |
| Accession | O43490 |
| Clone Number | S-R586 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu |
Background
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein that plays a role in cell signaling, cytoskeleton rearrangement, and protein trafficking, and is associated with stemness, cell proliferation, migration, and survival, particularly in the context of cancer stem cells and tumorigenesis.
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