Monoclonal Anti-DXD Antibody

ELISA analysis specificity of DXD mAb
Coating ADC: 1μg/ml
Add anti-DXD mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points
Add SA-HRP: 1:10000

Sandwich ELISA assay
Coating anti-DXD mAb: 5μg/ml
Sample: Trastuzumab deruxtecan (DXD ADC), from 12.5ng/ml, 2-fold diluent, 7 points
Add anti human Fc HRP: 1:4000

Fig.1. SDS-PAGE analysis of DXD_mAb003
Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)
Lane 1: Purified DXD_mAb003 under reducing condition (Sample loaded: 2 μg)
Lane 2: Purified DXD_mAb003 under non-reducing condition (Sample loaded: 2 μg)
• Recombinant protein was visualized by Coomassie Brilliant Blue R250 staining.

Fig. 2. SEC-HPLC analysis of DXD_mAb003
Running buffer: 2*PBS, pH 7.4
Column: Superdex 200 Increase 5/150 GL(GE, Code Number: 28-9909-45)

Fig.3. ELISA analysis of DXD mAb003
Coating DXD ADC: 1ug/ml
Add anti-DXD mAb: from 5ug/ml, 10-fold diluent, 8 points including 0
Add Anti-mouse IgG (Fc specific)-HRP: 1:5000

Fig.4. Sandwich ELISA analysis of DXD mAb003 detecting DXD-ADC
Coating Her2 ECD protein: 2ug/ml
Add DXD-ADC: from 10ug/ml, 10-fold diluent, 8 points including 0
Add DXD antibody mAb003 (mouse IgG) at 1ug/ml
Add anti-mouse IgG (Fc specific)-HRP: 1:5000

Fig.5. Sandwich ELISA analysis of DXD mAb003 detecting DXD-ADC
Coating anti-DXD mAb: 1ug/ml
Add DXD-ADC: from 10ug/ml, 10-fold diluent, 8 points including 0
Add anti-human IgG (Fc specific)-HRP: 1:5000