ELISA analysis specificity of DM1&DM4 mAb
Coating ADC: 1μg/ml
Add anti-DM1&DM4 mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points
Add SA-HRP: 1:10000
Product Details
Product Details
Product Specification
Clone Number | S-30C3 |
Application | ELISA |
Reactivity | Species Independent |
Concentration | Lot specific* (generally 1 to 5 mg/ml)* |
Molecular Weight | 50 and 25 kD in reducing condition, 150kD in non-reducing condition |
Purity | >95%SDS-PAGE & RP-HPLC |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Stability & Storage |
-80℃, avoiding
repeated freeze-thaw cycles
|
Picture
Picture
Validation Data


Sandwich ELISA assay
Coating anti-DM1&DM4 mAb: 5μg/ml
Sample: Trastuzumab emtansine (DM1 ADC), from 12.5ng/ml, 2-fold diluent, 7 points
Add anti human Fc HRP: 1:4000
SDS-PAGE

SEC-HPLC

ELISA





