Monkey Anti-PEG IgG ELISA
Monkey Anti-PEG IgG ELISA
Product Details
Product Details
Product Specification
| Usage |
1. Perform extraction as soon as possible after specimen collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, the specimens can be stored at -20°C, but repeated freezing and thawing should be avoided. 1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart.
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| General Notes | 1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not fully used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath during dilution can aid dissolution. Washing does not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably in duplicate. If the analyte content in the sample is too high (the OD value of the sample is greater than the OD value of the first standard well), dilute the sample a certain number of times (n) with sample diluent before measurement. When calculating the total dilution factor (×n×5), multiply the final dilution factor. 5. The sealing film is for single use only to prevent cross-contamination. 6. Please store the substrate in the dark. 7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious materials. 9. Components of different batches of this reagent must not be mixed. |
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| Storage Temp. | Unopened test kit, sealed and stored at 2-8℃, valid for 6 months | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Test Range | 40pg/ml –2200pg/ml |
