WB result of MG53 Recombinant Rabbit mAb
Primary antibody: MG53 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
MG53 Recombinant Rabbit mAb (S-1659-10)
MG53 Recombinant Rabbit mAb (S-1659-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MG53 |
| Synonyms | Tripartite motif-containing protein 72; Mitsugumin-53 (Mg53); TRIM72 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q6ZMU5 |
| Clone Number | S-1659-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HCT 116, mouse heart, rat heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
Background
MG53, also known as TRIM72, is a member of the TRIM protein family, primarily expressed in the heart and skeletal muscle. It consists of an N-terminal RING, B-box, and curly helix region, with a C-terminal SPRY domain. MG53 plays a crucial role in acute cell membrane repair, anti-inflammation, stem cell regeneration, and anti-virus effects. In acute cell membrane injury, MG53 oligomerizes and recruits intracellular vesicles to the injury site, forming membrane repair patches. It also inhibits the NF-κB pathway to reduce inflammation and promotes stem cell survival and regeneration. MG53 has shown therapeutic potential in myocardial infarction, acute lung injury, and other tissue injuries.
Picture
Picture
Western Blot
WB result of MG53 Recombinant Rabbit mAb
Primary antibody: MG53 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
WB result of MG53 Recombinant Rabbit mAb
Primary antibody: MG53 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
