WB result of Mesothelin Rabbit mAb Primary antibody: Mesothelin Rabbit mAb at 1/1000 dilution Lane 1: PC-3 whole cell lysate 20 µg Lane 2: HeLa whole cell lysate 20 µg Lane 3: SK-OV-3 whole cell lysate 20 µg Lane 4: OVCAR-3 whole cell lysate 20 µg Negative control: PC-3 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa Observed MW: 46~48, 69 kDa
Mesothelin Recombinant Rabbit mAb (SDT-347-15)
Mesothelin Recombinant Rabbit mAb (SDT-347-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Mesothelin |
| Synonyms | CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, MSLN |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q13421 |
| Clone Number | SDT-347-15 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC | 1:1000 |
Background
Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein normally expressed in mesothelial cells that line the pleura, peritoneum, and pericardium. The MSLN gene encodes a precursor protein of 71 kDa that is processed to a 31 kDa shed protein called MPF (megakaryocyte potentiating factor) and a 40 kDa membrane bound protein, mesothelin. Mesothelin is reported to be highly expressed in several types of malignant tumors, such as malignant mesothelioma, ovarian cancer, pancreatic adenocarcinoma, and lung adenocarcinoma.
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Western Blot
IP
Mesothelin Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Mesothelin in 0.4mg HeLa whole cell lysate. Western blot was performed on the immunoprecipitate using Mesothelin Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: HeLa whole cell lysate 20 µg (Input) Lane 2: Mesothelin Rabbit mAb IP in HeLa whole cell lysate Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate Predicted MW: 69 kDa Observed MW: 46~48,69 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
