WB result of MCM7 Recombinant Rabbit mAb
Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 81 kDa
MCM7 Recombinant Rabbit mAb (S-828-221)
MCM7 Recombinant Rabbit mAb (S-828-221)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MCM7 |
| Synonyms | DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P33993 |
| Clone Number | S-828-221 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, MCF7, HeLa, Jurkat, NIH/3T3, Neuro-2a, C2C12, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
MCM7, or minichromosome maintenance complex component 7, is a highly conserved protein that plays an essential role in the initiation of eukaryotic genome replication. MCM7 is part of the mini-chromosome maintenance proteins (MCM) family, which form a hexameric protein complex known as the pre-replication complex (pre-RC). This complex is crucial for the formation of replication forks and the recruitment of other DNA replication-related proteins. MCM7 has been found to associate with cyclin D1-dependent kinase, CDK4, which may regulate the binding of MCM7 with the tumor suppressor protein RB1/RB, impacting cell cycle progression. MCM7 is often overexpressed in various cancers and is considered a potential proliferation marker. Its high expression is associated with poor prognosis in hepatocellular carcinoma (HCC) and other types of cancer.
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Western Blot
WB result of MCM7 Recombinant Rabbit mAb
Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 81 kDa
WB result of MCM7 Recombinant Rabbit mAb
Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 81 kDa
