WB result of MCM6 Rabbit mAb
Primary antibody: MCM6 Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 93 kDa
Observed MW: 110 kDa
MCM6 Recombinant Rabbit mAb (S-899-23)
MCM6 Recombinant Rabbit mAb (S-899-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | DNA replication licensing factor MCM6, p105MCM |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q14566 |
| Clone Number | S-899-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:500 | null |
Background
MCM6 protein, also known as minichromosome maintenance protein 6, is a member of the MCM (minichromosome maintenance) family, which includes MCM2, MCM3, MCM4, MCM5, and MCM7. MCM6 protein is a crucial component of the pre-replication complex (pre-RC), which plays a vital role in DNA replication. Additionally, MCM6, along with other MCM family members, forms the MCM complex, which possesses DNA helicase activity and functions as a DNA helicase. In many cancer cells, MCM6 protein expression is enhanced. For example, the continuous elevation of MCM6 expression can promote the formation, development, and progression of hepatocellular carcinoma. Research has shown that upregulation or downregulation of MCM6 protein expression can regulate cell cycle, proliferation, metastasis, immune response, and the maintenance of the DNA replication system.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MCM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MCM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MCM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
