WB result of mCherry Mouse mAb
Primary antibody: mCherry Mouse mAb at 1/1000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
mCherry Mouse mAb (S-1050-13)
mCherry Mouse mAb (S-1050-13)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Synonyms | mCherry fluorescent protein |
| Immunogen | Recombinant Protein |
| Accession | X5DSL3 |
| Clone Number | S-1050-13 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | WB, FC |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICFCM | 1:2000 |
Background
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-mCherry-E-Tag transfected 293T (Human embryonic kidney epithelial cell) labelling CBP Tag antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Mouse IgG Isotype Control / (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
