WB result of Man6P Recombinant Rabbit mAb
Primary antibody: Man6P Recombinant Rabbit mAb at 1/250 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
Man6P Recombinant Rabbit mAb (S-2745)
Man6P Recombinant Rabbit mAb (S-2745)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Man6P |
| Synonyms | M6P; mannose 6-phosphate; N-glycosylation |
| Clone Number | S-2745 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human brain, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:250 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Mannose 6-phosphate (M6P) is a critical glycosylation marker primarily involved in the Golgi processing of lysosome-targeted proteins. After synthesis in the rough endoplasmic reticulum, the oligosaccharide chains of these proteins undergo phosphorylation to generate the M6P signal. This signal is then recognized and bound by M6P receptors (MPRs) on the Golgi membrane, facilitating vesicular transport to deliver the proteins to lysosomes. This mechanism is essential for the proper sorting of lysosomal enzymes. Dysfunction in this pathway (e.g., defective M6P generation or receptor mutations) can lead to lysosomal storage disorders, such as I-cell disease. The discovery of the M6P pathway has advanced the understanding of intracellular protein sorting and metabolic diseases, and it is also being explored for targeted delivery applications in gene therapy.
Picture
Picture
Western Blot
WB result of Man6P Recombinant Rabbit mAb
Primary antibody: Man6P Recombinant Rabbit mAb at 1/250 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Man6P antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
