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M13 OneStep ELISA Kit

M13 OneStep ELISA Kit

Catalog Number: S0C3033 Application: ELISA Reactivity: Other Conjugation: Brand: Starter
Price:
Regular price $500.00 USD
Regular price Sale price $500.00 USD
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Product Details

Product Specification


Antigen M13 Phage
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Others
Purification Protein A
Stability & Storage 2 to 8 °C as supplied.

Kit


Precision 板内变异系数 (Intra-assay): 6.1%;
板间变异系数 (Inter-assay): 7.5%
Sample type Cell culture supernatant
Assay type Sandwich (quantitative)
Sensitivity 7.079E+03 particles /ml
Range 2.205E+04 particles /ml – 2.500E+06 particles /ml
Recovery Cell culture supernatant: 92%
Assay time 60 minutes

Background

M13 is a filamentous phage that has attracted much attention in the field of molecular biology and biotechnology. It belongs to the family Inoviridae and mainly infects Escherichia coli (E. coli). M13 phage has a simple single-stranded DNA genome of about 6.4 kilobases and a rod-shaped morphology. M13 phage can display foreign peptides or proteins on its surface. By genetically engineering the M13 genome to include genes encoding proteins of interest in the phage coat protein genes, phage particles are generated that can display proteins on their surface. Phage titration ELISA is a technique used to determine the concentration or titer of phage (bacteriophage) particles in a given sample. The Phage Titration ELISA determines the relative number of phage particles and provides a rapid, sensitive and reproducible alternative method for titrating M13 phage preparations.

Picture

ELISA

Standard curve
Example of M13 standard curve in Assay Diluent #1.

Leading Competitor comparison

Protocol Diagram

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