M: marker ; Complement Luciferase Ⅰ，2μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%
Luciferase Ⅱ
Luciferase Ⅱ
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Product Details
Product Details
Product Specification
| Species | Photinus pyralis |
| Amino Acid Sequence | N.A |
| Expression System | E.coli |
| Molecular Weight | 61.7kDa |
| Purity | >95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris (pH7.4), 1 mM EDTA, 1 mM DTT, 50% glycerol |
| Reconstitution | |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81. 2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287. |
Background
Luciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. In our work, the luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, some biological applications are limited by the low stability of the luciferase, so we combined amino acid mutations to enhance the enzyme’s thermostability and was eventually named Luciferase Ⅱ.
Unit Definition
One unit of Luciferase is defined as the chemiluminescence module of PerkinElmer VICTROR Nivo was used to record the luminescence reading output within 5 minutes, namely the luminescence quantity CPS.
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SDS-PAGE
