WB result of LIN28 Rabbit mAb
Primary antibody: LIN28 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 26 kDa
LIN28 Recombinant Rabbit mAb (S-795-8)
LIN28 Recombinant Rabbit mAb (S-795-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9H9Z2 |
| Clone Number | S-795-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28 binds to the let-7 pre-microRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. In pluripotent embryonal carcinoma cells, LIN28 is localized in the ribosomes, P-bodies and stress granules. LIN28 is highly expressed in human embryonic stem cells[14] and can enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of iPS cells from human fibroblasts.
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Western Blot
IP
LIN28 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating LIN28 in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using LIN28 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: T-47D whole cell lysate 20 µg (Input)
Lane 2: LIN28 Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 23 kDa
Observed MW: 26 kDa
