2μg (R: reducing conditions, N: non-reducing conditions).
Leptin R/CD295 His Tag Protein, Human
Leptin R/CD295 His Tag Protein, Human
Price:
Regular price
$557.00 USD
Regular price
Sale price
$557.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | LEP-R, HuB219, OB receptor (OB-R), CD295 |
| Accession | P48357 |
| Amino Acid Sequence | Phe22-Asp839, with C-terminal 10*His FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDGGGSGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 100-120kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Bj?rbaek C, Uotani S, da Silva B, Flier JS. Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 1997;272:32686-32695. |
Background
Leptin cellular actions are mediated by leptin receptors (OB-Rs, Leptin R), which are membrane-anchored molecules binding to leptin and transducing its intracellular signal. Leptin R is encoded by the db gene. Leptin R is a member of cytokine receptor family that including various interleukins, interferon-γ, erythropoietin, growth hormone and prolactin. Proteolytic cleavage of transmembrane Leptin R produces a soluble leptin binding protein called soluble leptin receptor (sOB-R), which constitutes the major binding protein of circulating leptin in human plasma. The sOB-R exerts high affinity in binding leptin, antagonizing the membrane leptin receptors and, functionally, inactivating leptin. By binding circulating leptin, sOB-R modulates the bioavailability and efficacy of leptin, exerting a predominantly inhibitory effect on leptin signal transduction; thus, altering the sensitivity of target tissues to leptin actions and producing a state called leptin resistance in analogy to in sulin resistance. Genetic deficiency of either leptin or Leptin R causes severe obesity and obesity-associated diabetes.
Picture
Picture
SDS-PAGE
ELISA
Immobilized Leptin, Human (Cat. No. UA040148) at 10.0μg/mL (100μL/well) can bind Leptin R/CD295 His Tag, Human (Cat. No. UA010682) with EC50 of 0.96-1.29μg/mL.
