Measured in a cell proliferation assay using MCF-7 Human breast cancer cells. The EC50 for this effect is less than 2ng/ml
Leptin Protein, Mouse
Leptin Protein, Mouse
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$17.00 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Leptin, rRtLeptin, Obesity protein (OB), LEP |
| Amino Acid Sequence | Val22-Cys167 |
| Expression System | E.coli |
| Molecular Weight | 16kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 50mM NaCl，pH 8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Vitam Horm. 2005;71:345-72. |
Background
Leptin is the protein product encoded by the obese (ob) gene. It is a circulating hormone produced primarily by the adipose tissue, Which is an adipocyte-derived hormone that acts as a major regulator for food intake and energy homeostasis. Leptin deficiency or resistance can result in profound obesity, diabetes, and infertility in humans. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. It also plays important roles in angiogenesis, immune function, fertility, and bone formation.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).