Flow cytometric analysis of LAMP1 expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (Black line histogram) and SDT LAMP1 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
LAMP1 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-405-39)
LAMP1 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-405-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LAMP1 |
| Synonyms | Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; CD107a |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P11279 |
| Clone Number | SDT-405-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | Hu |
| ICFCM | 1:800 | Hu |
Background
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The LAMP-1 glycoprotein is a type I transmembrane protein [PMID: 16973206] which is expressed at high or medium levels in at least 76 different normal tissue cell types. It resides primarily across lysosomal membranes [PMID: 2584229], and functions to provide selectins with carbohydrate ligands. LAMP-1 has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells, and may also play a role in tumor cell differentiation and metastasis.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- LAMP1 (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
