IF shows positive staining in paraffin-embedded mouse spleen. Anti- Lamin B1 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with ED TA buffer pH9.0 was performed before commencing with IF staining protocol.
Lamin B1 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-307-108)
Lamin B1 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-307-108)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin B1 |
| Synonyms | LMNB1, Lamin-B1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus lamina |
| Accession | P20700 |
| Clone Number | SDT-307-108 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Mouse spleen, rat brain |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | Ms, Rat |
Background
Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. It is one of the essential members of the nuclear lamina protein family. Its main function is to maintain the integrity of nuclear skeleton, as well as to participate in the cell proliferation and aging by affecting the chromosome distribution. gene expression, and DNA damage repair. The abnormal expression of lamin B1 is related to certain diseases, including neurological diseases [e.g. neural tube defects (NDTs), adult-onset autosomal dominant leukodystrophy (ADLD)] and tumors (e.g. pancreatic cancer). It is also a potential tumor marker as well as drug target.
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded rat brain. Anti- Lamin B1 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti- Lamin B1 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with ED TA buffer pH9.0 was performed before commencing with IF staining protocol.
