WB result of Lamin A/C Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: Lamin A/C Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Predicted MW: 64, 74 kDa
Observed MW: 60, 74 kDa
Lamin A/C Recombinant Rabbit mAb (HRP Conjugate) (S-950-164)
Lamin A/C Recombinant Rabbit mAb (HRP Conjugate) (S-950-164)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin A/C |
| Synonyms | LMNA; LMN1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus lamina |
| Accession | P02545 |
| Clone Number | S-950-164 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rb, Rt, Bv, Dg, Gt |
| Positive Sample | HeLa, HUVEC, HepG2, NIH/3T3, C6 |
| Predicted Reactivity | Pg |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
Background
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome.
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Picture
Western Blot
WB result of Lamin A/C Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: Lamin A/C Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Predicted MW: 64, 74 kDa
Observed MW: 60, 74 kDa
WB result of Lamin A/C Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: Lamin A/C Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Predicted MW: 64, 74 kDa
Observed MW: 60, 74 kDa
