WB result of Lamin A Recombinant Rabbit mAb
Primary antibody: Lamin A Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 74 kDa
Observed MW: 68 kDa
Lamin A Recombinant Rabbit mAb (S-1503-44)
Lamin A Recombinant Rabbit mAb (S-1503-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin A |
| Synonyms | LMNA |
| Immunogen | Synthetic Peptide |
| Location | Nucleus lamina |
| Accession | P02545 |
| Clone Number | S-1503-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu |
| Positive Sample | A431, HeLa, PANC-1, MCF7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Lamin A is a type V intermediate filament protein essential for maintaining the integrity and mechanical properties of the nucleus. It is part of the nuclear lamina, which is a network of proteins beneath the inner nuclear membrane that helps determine the shape and size of the nucleus. Lamin A is involved in the regulation of gene expression and cell cycle progression. It interacts with the nuclear envelope and chromatin, influencing cellular processes such as DNA replication, cell cycle regulation, and signal transduction. Lamin A also serves as a "mechanosensor," linking the cytoskeleton and nuclear structures, and plays a role in the response to mechanical stress within the cell. Overexpression of Lamin A/C protein has been observed in invasive prostate cancer and is implicated in promoting cancer cell growth, migration, and invasion through the PI3K/AKT/PTEN pathway.
Picture
Picture
Western Blot
IP
Lamin A Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Lamin A in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Lamin A Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Lamin A Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 74 kDa
Observed MW: 74 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Lamin A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
