1μg (R: reducing condition, N: non-reducing condition).
LAG-3/CD223 His Tag Protein, Rat
LAG-3/CD223 His Tag Protein, Rat
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$236.00 USD
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Product Details
Product Details
Product Specification
| Species | Rat |
| Synonyms | Lymphocyte activation gene 3 protein，LAG3，Lymphocyte Activating 3，Lymphocyte-Activation Gene 3，CD223 Antigen，Protein FDC，CD223，LAG-3，FDC，sLAG-3 |
| Accession | Q5BK54 |
| Amino Acid Sequence | Gly24-Leu442 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 43-55kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE, ＞90% by SEC-HPLC &＞90% by RP-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Colin G. Graydon, Shifa Mohideen and Keith R.Fowke, LAG3’s Enigmatic Mechanism of Action. Frontiers in Immunology |
Background
LAG3, a member of the immunoglobulin superfamily and a homolog of CD4, arose from a gene duplication event. Similar to CD4, LAG3 binds to MHC class II (MHCII), but it also interacts with FGL-1, α-synuclein fibrils (α-syn), galectin-3 (Gal-3), and lymph node sinusoidal endothelial cell C-type lectin (LSECtin). As an immune checkpoint, LAG3 inhibits the activation of its host cell, promoting a more suppressive immune response. On T cells, LAG3 reduces the production of cytokines and granzymes, as well as cell proliferation, while encouraging differentiation into T regulatory cells.
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SDS-PAGE
SEC-HPLC
The purity of LAG-3/CD223 His Tag Protein, Rat is more than 90% determined by SEC-HPLC.
RP-HPLC
The purity of LAG-3/CD223 His Tag Protein, Rat is more than 90% determined by RP-HPLC.
ELISA
Immobilized LAG-3/CD223 His Tag, Rat (Cat. No. UA010786) at 2.0μg/mL (100μL/well) can bind Mouse FGL1 Protein, Fc Tag with EC50 of 0.082-0.107ug/mL.
