WB result of LacZ Recombinant Rabbit mAb
Primary antibody: LacZ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with E.coli LacZ-Myc-His fusion protein whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 40~130 kDa
LacZ Recombinant Rabbit mAb (S-1626-44)
LacZ Recombinant Rabbit mAb (S-1626-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LacZ |
| Synonyms | Beta-galactosidase; Beta-gal; Lactase |
| Immunogen | Recombinant Protein |
| Accession | P00722 |
| Clone Number | S-1626-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | E. coli |
| ICC | 1:500 | E. coli |
Background
LacZ, encoding for β-galactosidase, is a gene found in the lac operon of Escherichia coli. Its primary function is to catalyze the hydrolysis of lactose into glucose and galactose, providing a carbon and energy source for the bacteria. In molecular biology and genetic engineering, LacZ is widely used as a reporter gene due to its catalytic activity and ease of detection. It can break down a chromogenic substrate, X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), into galactose and a blue, insoluble product, which is useful for visualizing gene expression and distinguishing recombinant from non-recombinant clones in blue/white screening assays. Additionally, LacZ activity can be measured to study gene expression regulation mechanisms and levels.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in E.coli LacZ-Myc-His fusion protein transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- LacZ antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
