WB result of L-Lactate Dehydrogenase A/LDHA Rabbit pAb
Primary antibody: L-Lactate Dehydrogenase A/LDHA Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: RD whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
L-Lactate Dehydrogenase A/LDHA Rabbit Polyclonal Antibody
L-Lactate Dehydrogenase A/LDHA Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | L-Lactate Dehydrogenase A/LDHA |
| Synonyms | L-lactate dehydrogenase A chain; LDH-A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit (LDH-M); Renal carcinoma antigen NY-REN-59; PIG19 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P00338 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, HepG2, 293T, RD, mouse kidney, rat kidney |
| Predicted Reactivity | Cz, Or, CyMk, Rb, Op |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:200 | Hu, Ms |
Background
LDHA (Lactate Dehydrogenase A) is a key enzyme involved in glycolysis, catalyzing the conversion of pyruvate to lactate while regenerating NAD+ from NADH. This process is critical for maintaining cellular energy homeostasis, especially under anaerobic or hypoxic conditions. LDHA is highly expressed in various tissues and is a major component of the Warburg effect in cancer cells, promoting rapid glycolysis and lactate production to support tumor growth and survival. High levels of LDHA are associated with increased radiation resistance in tumors, making it a potential target for enhancing radiosensitivity. Additionally, LDHA expression is regulated by transcription factors such as c-MYC and SP1, which are modulated by proteins like TAB182, further influencing tumor metabolism and response to therapy.
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Western Blot
WB result of L-Lactate Dehydrogenase A/LDHA Rabbit pAb
Primary antibody: L-Lactate Dehydrogenase A/LDHA Rabbit pAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
WB result of L-Lactate Dehydrogenase A/LDHA Rabbit pAb
Primary antibody: L-Lactate Dehydrogenase A/LDHA Rabbit pAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded human breast cancer. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse kidney. Anti- L-Lactate Dehydrogenase A/LDHA antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
