IF shows positive staining in paraffin-embedded human colon cancer. Anti-Ki67 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Ki67 Recombinant Mouse mAb (Alexa Fluor® 594 Conjugate) (S-606-7)
Ki67 Recombinant Mouse mAb (Alexa Fluor® 594 Conjugate) (S-606-7)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Ki67 |
| Synonyms | Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, MKI67 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P46013 |
| Clone Number | S-606-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1 |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:100-1:200 | null |
Background
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy.
Picture
Picture
Immunofluorescence
