WB result of KDM5D Recombinant Rabbit mAb
Primary antibody: KDM5D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 174 kDa
KDM5D Recombinant Rabbit mAb (S-1577-128)
KDM5D Recombinant Rabbit mAb (S-1577-128)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KDM5D |
| Synonyms | Lysine-specific demethylase 5D; Histocompatibility Y antigen (H-Y); Histone demethylase JARID1D; Jumonji/ARID domain-containing protein 1D; Protein SmcY; [histone H3]-trimethyl-L-lysine(4) demethylase 5D; HY; HYA; JARID1D; KIAA0234; SMCY |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9BY66 |
| Clone Number | S-1577-128 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | TF-1 |
| Predicted Reactivity | Cz |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
KDM5D, also known as JARID1D or SMCY, is a gene located on the Y chromosome that encodes a protein with zinc finger domains. This protein acts as a histone demethylase, specifically targeting H3K4me2/3 marks, which are associated with transcriptional activation. KDM5D plays a role in various biological processes, including the development and function of the male reproductive system. It has been implicated in the regulation of genes that control cell adhesion and immune response in cancer, with its upregulation potentially contributing to cancer progression and gender differences in tumor behavior. KDM5D is also associated with minor histocompatibility antigens, which can lead to graft rejection in transplant settings. Its dysfunction has been linked to spermatogenic failure, highlighting its importance in male fertility.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-KDM5D antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-KDM5D antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-KDM5D antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
