Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (Human cervix adenocarcinoma epithelial cell) labelling mouse IgG2a isotype control at 1/200 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Invivo mouse IgG2a isotype control
Invivo mouse IgG2a isotype control
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | C1.18.4 |
| Antibody Type | Mouse mAb |
| Isotype | Mouse IgG2a, κ |
| Application | ELISA, FCM, In vivo control |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% Determined by SDS-PAGE |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| ICFCM | 1:200 | |
| FCM | 1:200 |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling mouse IgG2a isotype control at 1/200 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
