WB result of Invivo mouse IgG1 isotype control (D265A)
Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Invivo mouse IgG1 isotype control (D265A)
Invivo mouse IgG1 isotype control (D265A)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | SDT-844-79H(D265A)L |
| Antibody Type | Mouse mAb |
| Mutations | D265A |
| Application | WB, IHC-P, FC, In vivo control |
| Purification | Protein G |
| Concentration | Lot specific* (generally 5 to 20 mg/ml)* |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICFCM | 1:200 | |
| FCM | 1:200 |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
Western Blot
WB result of Invivo mouse IgG1 isotype control (D265A)
Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Invivo mouse IgG1 isotype control (D265A)
Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
FC
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling mouse IgG1 isotype control (D265A) at 1/200 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG1 isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling mouse IgG1 isotype control (D265A) at 1/200 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG1 isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows negative staining in paraffin-embedded human kidney. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human liver. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse spleen. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
