WB result of Invivo anti-mouse IL-2 mAb
Primary antibody: Invivo anti-mouse IL-2 mAb at 1/1000 dilution
Lane 1: IL-2 Protein, Mouse 1 µg
Lane 2: IL-2 Protein, Human 1 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 18 kDa
Product Details
Product Details
Product Specification
Host | Rat |
Antigen | IL-2 |
Synonyms | Interleukin-2; IL-2; T-cell growth factor (TCGF); Il2 |
Location | Secreted |
Accession | P04351 |
Clone Number | S-R536 |
Antibody Type | Rat mAb |
Isotype | Rat IgG2b,k |
Isotype Control | Invivo rat IgG2b isotype control |
Application | WB, in vivo IL-2 neutralization, in vitro IL-2 neutralization |
Reactivity | Ms |
Purification | Protein G |
Concentration | 5 mg/ml |
Endotoxin | <2EU/mg |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS pH7.4, containing no preservative |
Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
application | dilution | species |
WB | 1:1000 | Ms |
Background
Interleukin 2 (IL-2) is a cytokine that produced predominantly by Th cells, stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. IL-2 signals through a receptor complex consisting of the IL-2Rα subunit, IL-2Rβ subunit, and γc, which activates the JAK/STAT pathway, especially STAT3 and 5, leading to gene transcription.
Picture
Picture
Western Blot
