WB result of Invivo anti-mouse IFNγ Recombinant Rabbit mAb
Primary antibody: Invivo anti-mouse IFNγ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: IFN-γ Protein, Mouse 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 13 kDa
Invivo anti-mouse IFNγ Recombinant mAb
Invivo anti-mouse IFNγ Recombinant mAb
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Regular price
$150.00 USD
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$150.00 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | mouse IFNγ |
| Synonyms | Interferon gamma, IFN-gamma, Ifng |
| Accession | P01580 |
| Clone Number | S-R489 |
| Antibody Type | Recombinant mAb |
| Isotype | Rat IgG1,k |
| Application | WB, in vivo IFNγ neutralization, in vitro IFNγ neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | Lot specific* (generally 2 to 10 mg/ml)* |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
This antibody recognizes the same epitope as clone XMG1.2. Interferon-gamma (IFNγ) is a cytokine produced by activated T cells and natural killer (NK) cells, playing a crucial role in immune responses with various biological activities such as antiviral, anti-tumor, and immune-regulatory effects. IFNγ can induce the synthesis of indoleamine 2,3-dioxygenase, which is involved in the catabolism of tryptophan, and promote the production of nitric oxide (NO), potentially related to the occurrence or protective role in certain neurological diseases. Additionally, IFNγ is also a key player in tumor immunotherapy, as it can enhance the cytotoxic effects by promoting the migration of immune cells to the tumor microenvironment (TME), thereby limiting tumor growth.
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Western Blot
