WB result of CD29 Mouse mAb
Primary antibody: CD29 Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A-431 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 110~140 kDa
(This blot was developed with high sensitivity substrate)
Integrin beta1/CD29 Mouse mAb (S-933-228)
Integrin beta1/CD29 Mouse mAb (S-933-228)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Synonyms | Fibronectin receptor subunit beta, Glycoprotein IIa (GPIIA), VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P05556 |
| Clone Number | S-933-228 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, ICC, FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
CD29 is an integrin component that mediates adhesion and involves in homing to sites of inflammation. It expresses in fibroblasts, platelets, T cells, monocytes, granulocytes(low), mast cells, endothelial cells and myoepithelium, also other diverse cell types. It does not express in red blood cells and spermatogonia. It is a myoepithelial marker, although established markers (SMA, CD10, p63, S100, maspin, calponin, GFAP, smooth muscle myosin) are more commonly used.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of A549 (Human lung carcinoma epithelial cell) labelling CD29 antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti-CD29 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
