WB result of Integrin alpha V Recombinant Rabbit mAb
Primary antibody: Integrin alpha V Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 116 kDa
Observed MW: 130 kDa
Integrin alpha V Recombinant Rabbit mAb (S-1798-27)
Integrin alpha V Recombinant Rabbit mAb (S-1798-27)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Integrin alpha V |
| Synonyms | Integrin alpha-V; Vitronectin receptor; Vitronectin receptor subunit alpha; CD51; ITGAV; MSK8; VNRA; VTNR |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P06756 |
| Clone Number | S-1798-27 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A549, HT-29, HUVEC, RAW264.7, NIH/3T3, mouse kidney, mouse spleen, PC-12, C6, rat kidney, rat spleen |
| Predicted Reactivity | Bv, Ck |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Integrin alpha V (αV) is a subunit that pairs with various beta subunits to form different integrin heterodimers, each with distinct functions. Integrin αV plays a role in cell adhesion, migration, and signaling, particularly in processes such as angiogenesis, extracellular matrix (ECM) regulation, and cell survival. It binds to various ligands including vitronectin, fibronectin, osteopontin, and transforming growth factor-β (TGF-β). In the context of immune response, integrin αV has been implicated in the regulation of monocyte, macrophage, and neutrophil migration, as well as dendritic cell and macrophage phagocytosis, which are crucial for inflammation progression. Additionally, integrin αV has been associated with the promotion of local invasion and metastasis in neoplastic cells, highlighting its role in cancer progression. Overall, integrin αV is a key player in cellular interactions with the ECM and in the modulation of immune and cancer-related processes.
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Western Blot
WB result of Integrin alpha V Recombinant Rabbit mAb
Primary antibody: Integrin alpha V Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 116 kDa
Observed MW: 125 kDa
WB result of Integrin alpha V Recombinant Rabbit mAb
Primary antibody: Integrin alpha V Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Lane 4: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 116 kDa
Observed MW: 125 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Integrin alpha V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
