Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells in the presence of 10 ng/mL of recombinant human SCF. The EC50 for this effect is less than 0.2ng/ml.
IL-9 Protein, Human
IL-9 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P15248 |
| Amino Acid Sequence | Gln19-Ile144 |
| Expression System | E.coli |
| Molecular Weight | 15kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris,200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Randolph J Noelle and Elizabeth C Nowak (2010) Nat Rev Immunol. 10(10):683-7. |
Background
Nterleukin-9 (IL-9) was originally identified in mice as a T cell growth factor and is a member of the common γ-chain-receptor cytokine family, with other members including IL-2, IL-4, IL-7, IL-15 and IL-21. The IL-9 gene loci of both humans and mice have a similar organization, consisting of five exons, and share a 55% amino acid homology at the protein level. The IL-9 receptor consists of the cytokine-specific IL 9 receptor α-chain (IL-9Rα) and the γ-chain1. IL-9 induced receptor activation promotes the cross phosphorylation of Janus kinase 1 (JAK1) and JAK3. This cross-phosphorylation leads to the downstream activation of signal transducer and activator of transcription (STAT) complexes, specifically STAT1 homodimers, STAT5 homodimers and STAT1–STAT3 heterodimers. Although the contribution of IL-9 to the activation of these pathways in primary cells has not been fully elucidated, it may be of relevance in vivo.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
