Measured by its ability to inhibit IL-36β induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is less than 1μg/mL in the presence of 10ng/mL of recombinant human IL-36β.
IL-36Rα Protein, Human
IL-36Rα Protein, Human
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$176.00 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | FIL1 delta, IL-1 delta, IL-1F5, IL-1HY1, IL-1L1, IL-1RP3 |
| Accession | Q9UBH0 |
| Amino Acid Sequence | Val2-Asp155 |
| Expression System | E.coli |
| Molecular Weight | 13kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE&RP-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 300mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Li Zhou. (2021) Adv Exp Med Biol. 21:191-210. |
Background
The IL-36 family belongs to a larger IL-1 superfamily and consists of three agonists (IL-36α/β/γ), one antagonist (IL-36Ra), one cognate receptor (IL-36R) and one accessory protein (IL-1RAcP). The receptor activation follows a two-step mechanism in that the agonist first binds to IL-36R and the resulting binary complex recruits IL-1RAcP. Assembled ternary complex brings together intracellular TIR domains of receptors which activate downstream NF-κB and MAPK signaling. Antagonist IL-36Ra inhibits the signaling by binding to IL-36R and preventing recruitment of IL-1RAcP.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
